Journal Scans
Detection of EBV Genomes in Plasmablasts/Plasma Cells and Non-B Cells in the Blood of Most Patients With EBV Lymphoproliferative Disorders Using Immuno-FISH
Detection of EBV in Non-B Cells in Lymphoproliferative Disorders
S Calattini, I Sereti, P Scheinberg, H Kimura, RW Childs, JI Cohen
20100710
2010 Aug 10
Blood
Detection of EBV Genomes in Plasmablasts/Plasma Cells and Non-B Cells in the Blood of Most Patients With EBV Lymphoproliferative Disorders Using Immuno-FISH
Blood. 2010 Aug 10;[Epub Ahead of Print], S Calattini, I Sereti, P Scheinberg, H Kimura, RW Childs, JI Cohen
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Abstract
Abstract
Epstein-Barr virus (EBV) is present in B cells in the blood of healthy individuals; few studies have looked for EBV in other cell types in blood from patients with lymphoproliferative disorders. We employ a new technique combining immunofluorescent cell surface staining and fluorescent in situ hybridization, Immuno-FISH, to quantify both EBV copy number per cell and cell types in blood from patients with high EBV DNA loads. In addition to CD20+ B cells, EBV was present in plasmablast/plasma cells...
